The former Mr Simms Olde Sweet Shop in Ely High Street will be turned into a grocers and convenience store.
Estate agents Robinson Layer announced that it is the second store they have re-let in the High Street in recent weeks.
It comes as the former Anglia Wine shop opposite was let to a vaping firm last month.
Jonathan Lager of the agents, Robinson Layer, said: "This is the second retail unit at the top end of the High Street we have relet in recent weeks.
"So the High Street still is attractive to retailers."
The popular Mr Simms Olde Sweet Shop closed in August last year due to "business rates and fading footfall".
Dozens took to Facebook to express their sadness at the closure, with the announcement gaining more than 300 comments and over 100 shares.
The latest letting is a unit of 507 sq ft, located opposite the Lamb Hotel, at 5 Coronation Place, and is let on a five year lease at £17,000 per annum.
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here